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cyclin dependent kinase cdk 4  (Boster Bio)


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    Boster Bio cyclin dependent kinase cdk 4
    Cyclin Dependent Kinase Cdk 4, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cyclin+dependent+kinase+cdk+4/Anti-PCNA+Antibody/pmc11727511-58-34-40
    Average 95 stars, based on 292 article reviews
    cyclin dependent kinase cdk 4 - by Bioz Stars, 2026-09
    95/100 stars

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    Incubation:

    Article Title: Silencing LY6K Suppresses CD44 + EpCAM + HCT116 Human Colon Cancer Stem Cells Growth: Insights from In Vitro and In Vivo Evidence
    Article Snippet: .. The membranes were incubated overnight at 4 °C with primary antibodies [LY6K (1:750, #PIPA572689, Invitrogen), MMP-2 (3:1000, #BM0569, Boster, Pleasanton, CA, USA), MMP-9 (1:300, #BM4089, Boster), tissue inhibitor of MMPs-1 (TIMP-1) (3:1000, #BM4980, Boster), cyclin-dependent kinase (CDK) 4 (3:1000, #BM4672, Boster), cyclin D1 (3:1000, #bs-0623R, Bioss), CDK2 (3:1000, #bs-0757R, Bioss), cyclin E1 (3:1000, #bs-0573R, Bioss), caspase-3 and cleaved caspase-3 (1:1000, #14220, CST), β-actin (1:1500, #AF0003, Beyotime), and β-tubulin (1:1000, #AC008, Beyotime)], followed by incubation with secondary antibodies [horseradish peroxidase-conjugated goat anti-rabbit (1:2000, #A0208, Beyotime) and anti-mouse (1:2000, #A0216, Beyotime)] for 1 h at room temperature. .. The protein bands were visualized using super enhanced chemiluminescence detection reagent (Yeasen Biotechnology, Shanghai, China) by X-ray film and analyzed using Scion Image version 4.0.2 (Scion Corporation, Frederick, MD, USA).

    Article Title: Silencing LY6K Suppresses CD44 + EpCAM + HCT116 Human Colon Cancer Stem Cells Growth: Insights from In Vitro and In Vivo Evidence.
    Article Snippet: .. 2024, 46 14048 Boster), tissue inhibitor of MMPs-1 (TIMP-1) (3:1000, #BM4980, Boster), cyclin-dependent kinase (CDK) 4 (3:1000, #BM4672, Boster), cyclin D1 (3:1000, #bs-0623R, Bioss), CDK2 (3:1000, #bs-0757R, Bioss), cyclin E1 (3:1000, #bs-0573R, Bioss), caspase-3 and cleaved caspase3 (1:1000, #14220, CST), β-actin (1:1500, #AF0003, Beyotime), and β-tubulin (1:1000, #AC008, Beyotime)], followed by incubation with secondary antibodies [horseradish peroxidaseconjugated goat anti-rabbit (1:2000, #A0208, Beyotime) and anti-mouse (1:2000, #A0216, Beyotime)] for 1 h at room temperature. .. The protein bands were visualized using super enhanced chemiluminescence detection reagent (Yeasen Biotechnology, Shanghai, China) by X-ray film and analyzed using Scion Image version 4.0.2 (Scion Corporation, Frederick, MD, USA).



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    Image Search Results


    PCA combined with 5-FU induces the G1 phase cell cycle arrest in liver cancer cells. (A) Flow cytometry was carried out to evaluate the cell cycle distribution in HepG2, Huh7 and Huh1 cells treated with DMSO, 10 µM PCA, 10 µM 5-FU or their combination. Western blot analysis was performed to detect the protein expression levels of CDK4 and CDK6 in (B) HepG2, (C) Huh7 and (D) Huh1 cells treated with DMSO, 10 µM PCA, 10 µM 5-FU or their combination. Reverse transcription- quantitative PCR was performed to detect the mRNA levels of CDK4 and CDK6 in (E) HepG2, (F) Huh7 and (G) Huh1 cells treated with DMSO, 10 µM PCA, 10 µM 5-FU or their combination. * P<0.05 and ** P<0.01. PCA, purpurogallin carboxylic acid; 5-FU, 5-fluorouracil; CDK, cyclin-dependent kinase.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Purpurogallin carboxylic acid exhibits synergistic effects with 5‑fluorouracil on liver cancer cells in vitro by targeting ABCG2

    doi: 10.3892/etm.2024.12564

    Figure Lengend Snippet: PCA combined with 5-FU induces the G1 phase cell cycle arrest in liver cancer cells. (A) Flow cytometry was carried out to evaluate the cell cycle distribution in HepG2, Huh7 and Huh1 cells treated with DMSO, 10 µM PCA, 10 µM 5-FU or their combination. Western blot analysis was performed to detect the protein expression levels of CDK4 and CDK6 in (B) HepG2, (C) Huh7 and (D) Huh1 cells treated with DMSO, 10 µM PCA, 10 µM 5-FU or their combination. Reverse transcription- quantitative PCR was performed to detect the mRNA levels of CDK4 and CDK6 in (E) HepG2, (F) Huh7 and (G) Huh1 cells treated with DMSO, 10 µM PCA, 10 µM 5-FU or their combination. * P<0.05 and ** P<0.01. PCA, purpurogallin carboxylic acid; 5-FU, 5-fluorouracil; CDK, cyclin-dependent kinase.

    Article Snippet: Following blocking with 8% skim milk powder in TBS-Tween-20 (0.1%) (TBST) for 2 h at 28 ̊C, the membranes were incubated with primary antibodies against cyclin-dependent kinase (CDK) 4 (1:2,000; cat no. 11026-1-AP), CDK6 (1:1,000; cat no. 14052-1-AP), ABCG2 (1:1,000; cat no. 27286-1-AP) and GAPDH (1:50,000; cat no. 60004-1-Ig) all from Proteintech Group, Inc. for 16 h at 4 ̊C.

    Techniques: Flow Cytometry, Western Blot, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction